Journal: Scientific Reports

Article Title: Insight into the cellular fate and toxicity of aluminium adjuvants used in clinically approved human vaccinations

doi: 10.1038/srep31578

Figure Lengend Snippet: Representative lumogallion staining of agar-paraffin embedded (2 μm sections) THP-1 cells co-cultured with ( a ) 2.5, ( b ) 25.0, ( c ) 50.0 or ( d ) 100.0 μg/mL Alhydrogel ® (Brenntag Biosector, Denmark). Cell sections were incubated for 24 h in 100 μM lumogallion, 50 mM PIPES, pH 7.4. Slides were mounted with ProLong ® Gold Antifade Reagent with DAPI. All images depict lumogallion staining (orange) overlaid with DAPI-staining (blue). Magnified inserts show close-ups of individual cells with the light channel overlaid. White arrows highlight both individual and distinguishable adjuvant particles. Magnification X 1000, scale bars: 20 μm.

Article Snippet: Sections were finally air dried and mounted using ProLong ® Gold Antifade Reagent with 4′,6-diamidino-2-phenylindole, dihydrochloride (DAPI) (Life Technologies, UK) prior to storing horizontally for 24 h at 4 °C.

Techniques: Staining, Cell Culture, Incubation, Adjuvant

Journal: Scientific Reports

Article Title: Insight into the cellular fate and toxicity of aluminium adjuvants used in clinically approved human vaccinations

doi: 10.1038/srep31578

Figure Lengend Snippet: Representative lumogallion staining of agar-paraffin embedded (2 μm sections) THP-1 cells co-cultured with ( a ) 2.5, ( b ) 25, ( c ) 50 or ( d ) 100 μg/mL Adju-Phos ® (Brenntag Biosector, Denmark). Cell sections were incubated for 24 h in 100 μM lumogallion, 50 mM PIPES, pH 7.4. Slides were mounted with ProLong ® Gold Antifade Reagent with DAPI. All images depict lumogallion staining (orange) overlaid with DAPI-staining (blue). Magnified inserts show close-ups of individual cells with the light channel overlaid. White arrows highlight both individual and distinguishable adjuvant particles. Magnification X 1000, scale bars: 20 μm.

Article Snippet: Sections were finally air dried and mounted using ProLong ® Gold Antifade Reagent with 4′,6-diamidino-2-phenylindole, dihydrochloride (DAPI) (Life Technologies, UK) prior to storing horizontally for 24 h at 4 °C.

Techniques: Staining, Cell Culture, Incubation, Adjuvant

Journal: Scientific Reports

Article Title: Insight into the cellular fate and toxicity of aluminium adjuvants used in clinically approved human vaccinations

doi: 10.1038/srep31578

Figure Lengend Snippet: Representative lumogallion staining of agar-paraffin embedded (2 μm sections) THP-1 cells co-cultured with ( a ) 2.5, ( b ) 25, ( c ) 50 or ( d ) 100 μg/mL Imject™ Alum (Thermo Scientific). Cell sections were incubated for 24 h in 100 μM lumogallion, 50 mM PIPES, pH 7.4. Slides were mounted with ProLong ® Gold Antifade Reagent with DAPI. All images depict lumogallion staining (orange) overlaid with DAPI-staining (blue). Magnified inserts show close-ups of individual cells with the light channel overlaid. White arrows highlight both individual and distinguishable adjuvant particles. Magnification X 1000, scale bars: 20 μm.

Article Snippet: Sections were finally air dried and mounted using ProLong ® Gold Antifade Reagent with 4′,6-diamidino-2-phenylindole, dihydrochloride (DAPI) (Life Technologies, UK) prior to storing horizontally for 24 h at 4 °C.

Techniques: Staining, Cell Culture, Incubation, Adjuvant

Journal: Frontiers in Immunology

Article Title: Unveiling B7/CD28 family proteins in hepatocellular carcinoma: insights into communication and prognostic significance

doi: 10.3389/fimmu.2025.1583597

Figure Lengend Snippet: The expression pattern of B7H3 in tumor tissues. (A) The representative IHC images of Isotype control antibody and B7H3 antibody; (B) The representative IHC images of diffuse HCC and well-circumscribed HCC; (C) Representative multi-immunofluorescence images of AFP (green) and B7H3 (red). The nucleus is labeled with DAPI (blue). (D) Proportions of B7H3 on tumor cells (TCs), in stroma and in both. (E) The representative IHC images of B7H3 from serial sections. (F) OS and PFS for HCC patients based on the expression of B7H3 on tumor tissue. (G) OS and PFS for HCC patients based on the expression of B7H3 in the stroma and on tumor cells.

Article Snippet: In the end, the specimens were prepared by applying ProLongTM Diamond Antifade mountant with DAPI (G1236, Servicebio, Wuhan, China).

Techniques: Expressing, Control, Immunofluorescence, Labeling

Journal: Oncotarget

Article Title: Silencing of casein kinase 1 delta reduces migration and metastasis of triple negative breast cancer cells

doi: 10.18632/oncotarget.25738

Figure Lengend Snippet: ( A ) Scatter plot of gene expression analysis by RT 2 Profiler PCR Array in the shNT MDA-MB-231 cells compared to shCSNK1D cells. The central line indicates unchanged gene expression. ( B ) Taqman validation of genes identified in the RT Profiler array. The histogram represents fold change of genes up-or down-regulated in shCSNK1D compared to shNT control cells. ( C ) Western blot analysis of the expression of occludin (OCLN), junction adhesion molecule A (F11R) and claudin-1 (CLDN-1) in control (shNT) and inactivated cell lines (shCSNK1D). ( D ) Detection of OCLN and F11R by immunofluorescence in shCSNK1D compared to control shNT cells. Nuclei were stained with Dapi. Arrows indicate the increased expression of tight junction proteins.

Article Snippet: After washing, the cells were mounted with Prolong gold antifade medium with DAPI (Cell Signaling).

Techniques: Gene Expression, Biomarker Discovery, Control, Western Blot, Expressing, Immunofluorescence, Staining